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Composite
Part:BBa_K3286104
Designed by: Robert Hooftman Group: iGEM19_Wageningen_UR (2019-10-14)
Expression cassette for small proteins in E. coli BL21DE3
Use this part, containing a T7 promoter (BBa_K1614000), Bicistronic Design (BCD) system [1](BBa_M36516), Strep tag, MBP (Maltose Binding Protein) and TEV (Tomato Etch Virus) site (BBa_K3286103) to produce small protein domains in high volumes. After isolation of the fusion protein and treatment with TEV protease, run it again over a strep column to remove the Maltose binding protein.
[1] V. K. Mutalik et al., “Precise and reliable gene expression via standard transcription and translation initiation elements,” Nat. Methods, vol. 10, no. 4, pp. 354–360, Apr. 2013.
Sequence and Features
Subparts|Ruler|SS|DSScars: Show|HideVertical: Show|HideLength: UnknownGet part sequenceView plasmid
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Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 493
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 191
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Categories
Parameters
None |