Composite

Part:BBa_K3286104

Designed by: Robert Hooftman   Group: iGEM19_Wageningen_UR   (2019-10-14)


Expression cassette for small proteins in E. coli BL21DE3

Use this part, containing a T7 promoter (BBa_K1614000), Bicistronic Design (BCD) system [1](BBa_M36516), Strep tag, MBP (Maltose Binding Protein) and TEV (Tomato Etch Virus) site (BBa_K3286103) to produce small protein domains in high volumes. After isolation of the fusion protein and treatment with TEV protease, run it again over a strep column to remove the Maltose binding protein.

[1] V. K. Mutalik et al., “Precise and reliable gene expression via standard transcription and translation initiation elements,” Nat. Methods, vol. 10, no. 4, pp. 354–360, Apr. 2013.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 493
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 191


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Categories
Parameters
None